GagaVision No 41, which was posted on the singer's YouTube page on Wednesday, features behind the scenes footage of her tour as she prepares for a show.
The five-minute clip is the first episode of the web series - which Gaga made regular episodes of when she was promoting her debut album The Fame - since 2009.
However Gaga almost meets her match when she arrives at the concert venue and is confronted by a homophobic protestor.
The man gives her a business card which reads 'get out of hell free' and denounces her for what her refers to as her 'pervert ways'.
She also comments on his business card once she is in her dressing room.
'If it's so easy to get out of hell, why don't we just print up a bunch of these guys?' Gaga suggests, while adding, 'It makes me sad that my fans have to see that, but I know it's just part of what I do.'
Afterwards, the singer hits back by saying, 'There's 3000 people standing in my line - there's nobody in his line. Who's going to hell?'
She also comments on his business card once she is in her dressing room.
'If it's so easy to get out of hell, why don't we just print up a bunch of these guys?' Gaga suggests, while adding, 'It makes me sad that my fans have to see that, but I know it's just part of what I do.'
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